Cathepsin B is not the processing enzyme for mouse prorenin.

Twists and turns in the search for the elusive renin processing enzyme. Editorial Focus on “Cathepsin B is not the processing enzyme for mouse prorenin”

Prorenin processing by cathepsin B in vitro and in transfected cells.

Renin, which catalyzes the initial proteolytic cleavage reaction in the production of angiotensins, is first synthesized as a zymogen, prorenin, and requires the proteolytic removal of an amino-terminal prosegment for activation in vivo. The lysosomal hydrolase cathepsin B has been proposed as a prorenin processing enzyme based on reports of its co-localization with renin in the secretory granu...

Cathepsin B is not the processing enzyme for mouse prorenin Reudelhuber: Mouse prorenin processing enzyme

Renin, an aspartyl protease that catalyzes the rate-limiting step in the renin angiotensin system (RAS), is proteolytically activated by a second protease (referred to as the prorenin processing enzyme or PPE) before its secretion from the juxtaglomerular (JG) cells of the kidney. Although several enzymes are capable of activating renin in vitro, the leading candidate for the PPE in the kidney ...

Hydrolysis by cathepsin B of fluorescent peptides derived from human prorenin.

Cathepsin B is a lysosomal thiolprotease that, because of its colocalization with renin and its ability to activate prorenin, has been proposed as a prorenin processing enzyme. To characterize the biochemical aspect of this potential cathepsin B activity in more detail, we synthesized and assayed with human cathepsin B the internally quenched fluorescent peptide Abz-FSQPMKRLTLGNTTQ-EDDnp (Abz, ...

Twists and turns in the search for the elusive renin processing enzyme: focus on "Cathepsin B is not the processing enzyme for mouse prorenin".

HAVING WORKED FOR MANY YEARS on relatively basic questions germane to the venerable renin-angiotensin system, we were musing with some fascination recently on the continuing evolution of our understanding of the system. In particular, we were taking note of the considerable amount of dogma, with varying levels of verification associated with the system, as well as the almost continuous infusion...